Culturing yeast and using slants.
Information contained here was retrieved from various internet sources, such
as the Home Brew Digest, the Usenet newsgroup rec.crafts.brewing, and the
Yeast.FAQ, compiled by Patrick Weix and available via anonymous FTP at the
Sierra homebrew archive. Particular acknowledgement is due to Jack
Schmidling, whose r.c.b. post on this subject motivated me to finally get
going on doing this. Express permission is given for this information to be
used anytime, anywhere, by anyone, for any purpose. Note: I use metric units
throughout this document--sorry, you're just going to have to live with it!
Some of what you gain from doing slants is a) indefinite (practically
speaking) storage, b) assured maintenance of the original generation, and c)
ease of sharing yeast with brewpals. Also, when it comes time to make up a
starter to pitch into a batch of beer, you get 500 ml of active starter within
24-36 hours every time. Finally, if you are doing slants, then when someone
sends you a sample of yeast, or you get one from some other means, you can
make yourself a renewable lifetime supply from that sample.
II. Equipment you need
- A bunch of glass vials or test tubes that have caps that can a)
withstand temps of 100 deg. C and b) form a tight seal. I started with
30, and I think that is plenty, because as you proceed, using the yeast
in them, you just reculture into the spent ones as necessary. I use
flat-bottomed glass vials, capacity about 50 ml, because they are easier to
handle--you don't need a test tube rack. I got them here in Sydney--it's a
long, odd story--but you should be able to get them from some kind of
scientific supply outlet. Check the yellow pages under "laboratory
equipment". My 30 vials + caps cost me A$25, roughly 80 cents per unit.
- A dish made of something like pyrex, that can also withstand boiling
temperatures without exploding!
- Something to use for your starter vessel, like an old-style milk bottle,
an Erlenmeyer flask (that's what I use), or other glass vessel that has a
mouth to which you can affix a rubber-stopper + airlock.
The other hardware you will already have if you brew beer: a scale, big pot to
boil in, kitchen stove, refrigerator, spoons, etc etc etc.
III. Materials you need
- Either gelatin (Davis gelatin from Woolworths and other fine
supermarkets costs about 90 cents for a box of 5 packets) or agar-agar,
available at some Asian food stores. This is the growth medium. I use
gelatin and it works just fine--easy, cheap, always available.
- A bag of dried malt extract. One bag will last you the rest of your
life as far as keeping a full supply of yeast slants on hand is
- A bit of ethyl alcohol. 250 ml will last you for years of culturing
IV. Preparing "blank" slants
Now, the assumption here is that you are culturing from a pure source, like a
Wyeast pack, one of your bottled beers, or a slant that someone sends you. If
you are culturing, say, from a bottle of commercial bottle-conditioned beer,
extra steps are required to isolate pure cultures (bottling strains are rarely
pure). This is much more involved, I have never done it, and have no
intention to any time soon, so I will skip it here.
The first step is to make up a bunch of slants to use. It is very easy:
- Bring 1 cup (about 250 ml) water to a boil. Remove from heat, add 15 grams
of dried malt extract, and stir till dissolved. Put back on the heat and boil
for 5-10 minutes to ensure sterility. Remove from heat.
- Pour a packet of gelatin into this "wort" and stir till COMPLETELY
dissolved. Now pour this mixture into as many of your vials/test-tubes as you
can; a small funnel is useful for this step. Fill the vials about 1/4 full--
do NOT fill them all the way up. Keep at least one vial empty for use in the
next stage--see below. I typically fill about 15-20 vials with these amounts.
- Now place the pyrex dish mentioned above inside a large pot that has a lid
(like your brewkettle). Place your partially-filled slant vials in the dish.
Here is one place where having the flat-bottomed vials makes life easy--just
stand them up in the dish. (If you have test-tubes, you will need a rack to
stand them in, and the rack must sit in the pyrex dish. If your rack is the
right shape and size, you may be able to omit the pyrex dish.) Put a couple
cm of water in there--be sure it doesn't come up over the lip of the pyrex
dish. Crank up the heat so that this water boils (full power will probably
not be required), and keep it boiling for 10-15 minutes. If you wish, you can
put the vial-caps in there too, or just sterilize them with your favorite
chemical agent--doesn't matter. My usual procedure is to just toss the vial
caps into the water that is boiling. What is happening at this stage is that
the steam from the boiling water is sterilizing the vials, the growth medium,
and the caps (if they are in there too).
- Now turn off the heat, and have a couple homebrews while it cools off.
What you have at this point is sterilized vials + liquid growth medium. You
need to wait for things to cool to at least 40 deg. C before attaching the
sterile caps, otherwise the cooling growth-medium will cause the vials to
either suck the caps into the vials, or actually implode. Once cool enough,
put the caps on the vials firmly. You are now out of the woods as far as
sanitation is concerned.
- Now you must cool the vials while placing them at an angle of about 40-45
degrees. I do this by standing the vials in a box-lid (one with a good tall
lip around it, like the box-top to a box of xerox paper), and stacking a bunch
of oddments under one end, holding that end up, at the appropriate angle.
When you do this, the surface of the still-liquid-but-cooling gelatin + malt
extract will of course stay horizontal. Let the vials sit like this for 24
hours, after which time the gelatin + malt will be as solid as it gets (which
is still a bit soft and yielding--ideal for this purpose). After cooling, the
surface of the medium is at the angle you propped the box-top up to--that is,
it's "slanted", hence the name. These are now ready to be "inoculated" with
cells of your favorite yeast.
The time to do these 5 steps is no more than an hour (depending on how many
homebrews you have :-}).
V. Inoculating the blank slants with yeast
OK, so now you have a bunch of slants. At this point, the
procedure depends on what the source is for your yeast to be cultured.
I'll describe doing it from a packet of Wyeast, and then comment on
variations used for other sources.
Culturing your slants from a Wyeast pack
Before you start, you should lay out your working area in an organized way
to minimize having to get up and down, reach long distances for things,
etc. Sort out your worldly affairs before beginning--i.e., take a leak,
feed the dog, take out the rubbish. Then wash your hands thoroughly, and
begin. Have your Wyeast pack, slant vials, an unwrapped paper clip or
long needle, a cotton ball or folded up paper-towel, your vial of ethyl
alcohol, and your starter vessel laid out on clean paper toweling, along
with an empty, unused slant vial that has been sterilized, along with its
- As per usual procedure, get your Wyeast ready to make a starter, i.e. by
breaking the inner packet and waiting a day or two for it to swell up.
Make up a 1040 wort, cool it, and add it to the starter vessel, being sure
to aerate it well, as usual. Loosely sit its airlock in place--you want
it to be easy to remove with one hand in the Step 2.
- Stop breathing. Shake the Wyeast pack well, and then open it using
standard procedures. Pour a tiny bit of the Wyeast solution into your empty
and waiting vial, and pour the rest into your starter. Cap your vial, and
attach your airlock to your starter. Resume breathing. Your starter will be
ready to use the in the batch of beer you will brew tomorrow; the small
quantity of solution will be used to inoculate slants.
- Now repeat the following sequence of actions for each slant vial you wish
to inoculate. First, take a deep breath and hold it. Wipe your
needle/paperclip with alcohol-moistened cotton/paper towel to sterilize it.
Open the slant vial to be inoculated. Then open the vial with the bit of
Wyeast solution in it. Dip your needle into the Wyeast, and then lightly
poke it into the surface of the gelatin + malt growth medium in the slant
vial. Poke it in a bunch of places. Smear it around. Just try hard not to
touch the walls of the vial. When done, withdraw the needle, cap the slant
vial, and cap the Wyeast vial. Exhale. You now have a fully inoculated slant
vial. Keep doing this for as many vials as you wish to inoculate (see
The total amount of time to do these 3 steps is about 20 minutes for 10
slants, maybe a little more the first time you do it, till you develop the
- When done, leave the vials out at room temperature (20 deg C) for a week.
Within a couple of days you will see a cloudy film on the slant surface, and a
few days later it will develop into a milky white layer about a mm thick.
Sometimes, depending on the strain of yeast involved, the ambient temperature,
and the richness of your growth medium, the CO2 evolved from the yeast growing
on the slant surface may begin to push the cap up off the vial. No big deal--
just bleed the gas out by cracking open the cap for a moment, and press the
cap back down firmly. After the week is over, wrap the tops of the vials
(where the caps meet the vial walls) with electrician's tape, and put the
vials into a ziplock bag and pop them in the fridge, where they will keep for
at least 3 months in a perfectly viable condition. Obviously, if you are
keeping more than one yeast type around, you will want to label the vials
somehow--masking tape works perfectly for this.
VI. General comments
- How many vials should you inoculate at a time? I usually do 3 or 4 vials
of a given yeast type in a session, because I rarely use the same yeast 4
times in 3 months. When 3 months is nearly over, I simply reculture the
strain by doing the above to 3 or 4 new slants, but using an "old" slant as
the source instead of a vial with a few ml of Wyeast solution in it.
Otherwise the procedure is identical. Reculturing in this way does not
increment the strain generation-number because the yeast have not made enough
copies of themselves for mutation to occur.
- If culturing onto slants from a bottled-beer source, I would make
a small-volume starter and wait till that is fully active, then pour
off most of the liquid, swirl around what is left, and put several ml
of that into the empty vial as the source. WARNING! Many types of
bottle-conditioned beer use either a different kind of yeast in the
bottle than they fermented the beer with, or it is the same kind but
has mutated, or any of several other possible complications. If you
culture from a bottle of commercial beer, taste the small
starter you make from it when inoculating your slants. If it tastes
good (or at least, not bad) you are probably OK. But you should still
test the yeast on a small, pilot batch of beer before committing your
entire batch to it. Even when using a bottle of your own homebrew,
things can happen, so I would recommend these cautions in that case as
well. Caveat brewor.
- Making a starter from a slant: Before starting, be sure to let your slant
sit out for an hour or two so it can SLOWLY come up to room T from fridge T.
Now make a starter as usual. After boiling up your starter mini-wort (I
usually use a 1040 wort, which I get by boiling up 50 gr of dried malt extract
in 500 ml water), cool it, and then pour a bit into your slant vial (a
sanitized funnel is useful here too). Swirl it around to loosen as much yeast
as you can. Usually not all the yeast comes off the slant surface by simple
swirling, so have your needle and alcohol ready in this case. You just wipe
the needle sterile with the alcohol, and then gently scrape the slant surface
till the yeast comes off (don't worry if some of the gelatin tears loose--
doesn't matter). Then pour that into your starter vessel (along with the rest
of the mini-wort of course) and attach the airlock. At 20-22 deg. C, the
amount of yeast on a typical slant will produce a vigorously working starter
in 24-36 hours; a little more time is required at cooler temperatures. When
pitching the yeast from the starter, pour in most of the liquid part, and then
swirl your starter vessel to ensure that all the yeast cells that collect at
the bottom of the vessel are suspended in the remaining liquid; then dump that
into your fermenter as well. In my brewing, starters produced in this way
give good starts--I've never seen a lag time longer than 12-15 hours (in
cooler weather), with 8 hours being the norm. As always, be sure to
aerate both your starter wort and your main batch before adding any yeast.
- Cleaning the slant vials: If you use gelatin, hot water will dissolve
it after you have used the yeast to make a starter. Just add some, swirl,
and dump out over and over until the gelatin is dissolved away. Don't
worry too much about making them spotless, because they will be sterilized
next time you make a batch of blanks. I've never used agar-agar, and
expect that slightly more involved methods are required to clean it out of
- Things that can Go Wrong: The most common problem in making your own
slants is that mold can take hold on the slant surface. When this happens, it
is absolutely unmistakeable--a big greenish fuzzy patch just grows like
wildfire. Simply ditch that slant--it is hopeless. This is why you should
always make at least three new slants when propagating from an "old" one--so
that you will be more likely to have one pure one if things go wrong. It is
also possible for bacteria to get a foothold too. In my experience, this has
not happened so far. At first, I tasted every starter I made to be sure, but
after 50 times with no problems I gave up doing this. As long as the slant
surface looks "good"--i.e., a nice milky layer of yeast, no other colors or
shapes--you should be fine.
OK, so it took me 4 pages to write all this--I only wanted to be as verbose as
possible (and with me, that's pretty damn verbose) so that there will be as
few unanswered questions as possible. The amount of time expended to have
endless pure yeast on hand is a couple hours per strain, and for me, there is
no other way to go. I have spent $ on six Wyeast packs (1056 American, 1084
Irish, 2112 California lager, 1338 European Ale, 2565 Kolsch, and 1028 London
Ale), and now it is yeast forever, world without end, amen--bringing my cost
per batch for yeast very quickly to essentially zero. And hey--I LIKE being a
yeast rancher! I look in on 'em all the time, talk to 'em ("Hi boys, I'm
David Draper, Sydney, Australia, January 1995
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Spencer W. Thomas (firstname.lastname@example.org)